Class Topic
DATE | EXPERIMENT | EXERCISE |
JANUARY 28 | 1, DAY 1 | Genotype/Phenotype (shift cultures and frog) |
FEBRUARY 4 | 1, DAY 8 | (UV kill curves, alpha factor arrest) |
FEBRUARY 11 | 2, DAY 1 | Mating/Linkage (set up mating), Computer tutorial |
FEBRUARY 18 | 2, DAY 8 | (dissection and frogging) |
FEBRUARY 25 | 3, DAY 1 | Cloning by complementation (transformation of strain) |
MARCH 4 | 3, DAY 8 | (smash and grab) |
MARCH 6 (THURSDAY) | 3, DAY 10 | (miniprep, digest DNA) |
MARCH 11 | Spring Break | |
MARCH 18 | Spring Break | |
MARCH 25 | 4A, DAY 1 | Purify PCNA (grow cells, induce, collect) |
APRIL 1 | 4A, DAY 8 | (put onto column) |
APRIL 8 | 4B, DAY 1 | Western Blot (run gel and transfer) |
APRIL 15 | 4B, DAY 8 | (wash blot and develop) |
APRIL 22 | 5 | Class Test, Exp. 5 |
APRIL 29 | 5 | Exp. 5 |
MAY 6 | 5 | Exp. 5 |